Air-liquid interface (ALI) exposure as an alternative method to mimic human inhalation of airborne substances

Epidemiological and animal studies are both widely used approaches to investigate potential adverse effects and conduct risk assessments on inhaled chemicals and drugs. However, results are not always easy to interpret or reproduce and such experiments are extremely expensive, time consuming and use large numbers of animals. For these reasons, there is an increasing demand for the development of alternative approaches that make use of reliable in-vitro testing strategies. These testing methods will require realistic lung cell models, realistic inhalation exposure systems and proper dosimetry techniques to increase the predictive ability of in-vitro cell models and therefore accelerate the shift from in-vivo towards in-vitro testing.

In this poster presentation, we will show how ALI exposure works, give an overview of commercial systems, including the ALI module co-invented by VITO and will present cases using the ALI platform.


Evelien Frijns
Jo Van Laer
Karen Hollanders,
An Jacobs
Sandra Verstraelen


Flemish Institute for Technological Research (VITO NV), Boeretang 200, 2400 Mol, Belgium

Presenting author

Sandra Vanstraelen, Researcher, VITO
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